Iodide in urine

Standard addition analysis of iodide in diluted (factor 10) and filtrated urine using ECD with a silver working electrode (20 μL).

Iodide in Urine

• Extremely selective Ag electrode
• Quick and easy sample prep
• Robust & reproducible

A method for the determination of iodide is developed using electrochemical detection (ECD) with a silver working electrode (WE). The method consists of ion exchange chromatography using a phosphate/citrate buffer at pH 6.5, followed by amperometric detection at 0.15 V.

Detection limit of iodide is 0.2-1 μmol/L (25 - 127 ng/mL) depending on the column performance. Reproducibility of the determination of iodide is concentration depended. At 50 μM RSD in peak area's and heights was better than 2%. Below 1 μM the RSD values increased to 10 – 22 %.

System configuration

For the analysis of Iodide we selected the Flexcell with silver working electrode. The cell as a virtually maintenance free HyREF reference electrode.

The ALEXYS system comprises the following parts:

• AS 110 autosampler, cool
• LC 110S HPLC pump
• DECADE II electrochemical detector, SDC
• Flexcell with Ag WE and HyREF
• OR 110 organiser rack
• LC connection kit
• USB connection kit, 3 port
• Clarity data acquisition software

For more details see our product catalogue.

Notes

• 214_007_02 - Compilation of Clinical Applications