Left: a blank, 2 nM and 10 nM ACh and Ch standard (5 µL). Right: 100 fmol on column, different concentrations and injection volumes.
The Acetylcholine Analyzer has been developed for the analysis of acetylcholine and choline. Both are analysed by micro HPLC with an Immobilised Enzyme Reactor (IMER). The method is optimised for analysis of small sample volumes at low nanomolar detection limit.
An additional Choline Oxidase IMER is available to either suppress the Choline peak (pre-column placement) or detect Choline only (used as post-column IMER). A Choline Oxidase/Catalase IMER is available to convert Choline (ChOx) and to oxidise the released H2O2 (Catalase) to completely suppress the Choline response.
The ALEXYS micro LC-EC system uses a Flexcell with easily exchangeable working electrode disks in combination with the ACh/Ch assay kit and peroxidase kit of BASi. The ALEXYS system comprises the following parts:
For more details see product catalogue.
|180.0065C||ALEXYS Acetylcholine Analyzer|