Aminoglycoside antibiotics

Two examples of aminoglycoside analysis. Overlay of Spectinomycin (left, n=6) and Gentamycin C1/C2 (right, n=7)..

ALEXYS Aminoglycoside Analyzers

• Flexcell with exchangeable electrode
• Reproducible analysis of product impurities
• In accordance with Pharmacopeia methods*

The Aminoglycoside Analyzers are a number of system solutions for the analysis of Neomycin, Framycetin, Tobramycin, Lincomycin, Gentamicin, Spectinomycin, Kanamycin and Amikacin. The system is dedicated to the analysis of these antibiotics in pharmaceutical formulations and bulk product. Aminoglycosides are manufactured by a fermentation process and the main constituents as well as impurities and by-products can be identified and quantified.

The  system uses reversed phase chromatography with a post column addition of sodium hydroxide. Except for the Kanamycin/Amikacin analyzer which uses an ion exchange column.

* In a few cases the USP/EP conditions were modified, to improve assay specifications regarding reproducibility and peak resolution. 

System configuration

For the analysis of aminoglycosides we selected the Flexcell electrochemical cell. This cell has an exchangeable working electrode which is particularly useful in pulsed electrochemical detection. The electrode material tends to vanish over the years, in the process of pulsed amperometric detection. Exchanging an electrode disc only takes a few minutes. The HyREF is completely maintenance free even at pH 13 which is required for pulsed amperometric detection! The ALEXYS system comprises the following parts:

• AS 110 autosampler, cool
• LC 110S HPLC pump (2)
• OR 110 organiser
• Clarity data acquisition software
• DECADE II electrochemical detector
• Flexcell with Au WE, HyREF

Aminoglycosides 180.0050/56/59 require post-column addition of sodium hydroxide for detection. System 180.0058C is based on ion exchange chromatography at high pH (no post column addition). In addition to the first mentioned list this system includes Helium-proof solvent bottles. 

Notes

• 217_010_10 - Neomycin and Framycetin sulfate in bulk drug
• 217_013_09 - Gentamicin Sulphate in Pharmaceutical Preparations
• 217_014_06 - Tobramycin in Pharmaceutical Preparations
• 217_015_06 - Amikacin and Kanamycin in Bulk Drugs
• 217_018_07 - Spectinomycin in Pharmaceutical Preparations
• 217_019_05 - Spectinomycin and Lincomycin

Posters

• 221_005_03 - Aminoglycoside Antibiotics
• 221_016_01 - Spectinomycin and Lincomycin
• 221_017_02 - Macrolide and Aminoglycoside Antibiotics analyzer