Azithromycin

Analysis of Azithromycin, Clarithromycin, Erythromycin, Roxithromycin and Azaerythromycin.

ALEXYS Azithromycin Analyzer

• Excellent reproducibility and linearity
• In compliance with USP requirements
• Total analysis time <14 min

An ALEXYS system has been developed as a system solution for the analysis of Azithromycin following the official USP method. The USP method for Azi uses two flow cells in series, cell 1 for screening and cell 2 for detection of the analytes. The first flow cell is a Reactor cell, the second is the VT-03 cell. Under the specified LC conditions all USP criteria are met.

A mixture of several macrolides was analysed to illustrate the chromatographic performance of the method. All components are separated with at least a resolution of 2.5.

System configuration

For the analysis of Azythromycin we selected the VT03 electrochemical cell. This cell not only has an excellent reproducibility but also the best possible detection sensitivity. A Reactor was used as pre-oxidation screening cell to meet USP requirements. The ALG is a USP L49 type of column.

The ALEXYS system comprises the following parts:

• AS 110 autosampler, cool
• LC 110S HPLC pump
• OR 110 organiser rack
• Clarity data acquisition software
• DECADE II electrochemical detector, DCC
• VT cell with GC WE, sb REF
• Flexcell with GC WE and HyREF

Notes

• 217_017_07 - Azithromycin, Erythromycin and other Macrolide Antibiotics

Posters

• 221_018_01 - Azithromycin in pharmaceutical dosage forms