Glutathione and disulfides

ALEXYS Disulfides Analyzer

• Both mono- and disulfides in one run
• Detection limit 20 fmol on column (10 nM/2µL)

The Disulfides Analyzer has been developed for the analysis of (di-) sulfides such as (di-)glutathione, cystine, cysteine, homocystine, and homocysteine. The method applies a Flexcell with the new Magic Diamond working electrode for detection of mono and disulfides.

The chromatogram on the right shows the analysis of 2 µL, 1 µmol/L cystine (1), cysteine (2), glutathione (3), homocysteine (4) and diglutathione (5). 

System Configuration

For the analysis of disulfides we selected the Flexcell electrochemical cell. This cell has an exchangeable working electrode and a virtually maintenance free HyREF. The ALEXYS system comprises the following parts:

• AS 110 autosampler, cool
• LC 110S HPLC pump
• OR 110 organiser rack
• Clarity data acquisition software
• DECADE II electrochemical detector SCC
• Flexcell with MD WE, HyREF

For more details see our product catalogue.

Notes

• 211_004_04 - Glutathione and Other Disulfides

Posters

• 221_020_02 - Disulfides with Magic Diamond Electrode
• 221_003_02 - Homocysteine in plasma