Glutathione and disulfides

ALEXYS Disulfides Analyzer

• Both mono- and disulfides in one run
• Detection limit 20 fmol on column (10 nmol/L, 2µL)

The Disulfides Analyzer has been developed for the analysis of (di-) sulfides such as (di-)glutathione, cystine, cysteine, homocystine, and homocysteine. The method applies a Flexcell with the new Magic Diamond working electrode for detection of mono and disulfides. Detection limits are below 0.1 µmol/L for most (di-)sulfides. The chromatogram on the right shows the analysis of 2 µL, 1 µmol/L cystine (1), cysteine (2), glutathione (3), homocysteine (4) and diglutathione (5). 

If only the mono-sulfides are of interest, the Flexcell can be equipped with a Gold working electrode. This offers additional selectivity, and the lower working potential results in a better detection limit as well (10 nmol/L). Only if detection sensitivity for the di-sulfides on MD is an issue, they can be detected on a gold working electrode as well using a pre-reduction reactor cell (dual cell configuration).

System Configuration

For the analysis of disulfides we selected the Flexcell electrochemical cell. This cell has an exchangeable working electrode and a virtually maintenance free HyREF. The ALEXYS system comprises the following parts:

• AS 110 autosampler, cool
• LC 110S HPLC pump
• OR 110 organiser rack
• Clarity data acquisition software
• DECADE II MD electrochemical detector SCC
• Flexcell with MD WE, HyREF

or (high sensitivity DCC method only):

• DECADE II electrochemical detector DCC
• 2 Flexcells with GC and Gold WE, HyREF

For more details see our product catalogue.

Notes

• 211_004_04 - Glutathione and Other Disulfides

Posters

• 221_020_02 - Disulfides with Magic Diamond Electrode
• 221_003_02 - Homocysteine in plasma