µPrepCell

µ-PrepCell™ synthesizer for metabolites

• Synthesizer for metabolites
• Quick and clean alternative for in-vivo studies
• Synthesize micrograms of metabolites
• Collect fractions for MS or NMR
• High efficiency conversion

Structural elucidation of reactive metabolites plays an important role in drug development. Xenobiotics such as drugs are often metabolized by cytochrome P450 enzymes (CYP) to oxidative metabolites. A number of publications have shown that the vivo formation of these metabolites can be mimicked in vitro by electrochemical oxidation in a flow cell. Generating these metabolites in vitro has a number of advantages over in vivo formation, most important: it is much quicker and cleaner. Metabolites are formed instantaneously in a well defined matrix.

The µ-PrepCell is designed to synthesize micrograms of metabolites in a short period of time. The active surface of the working electrode is about 10 times the size of a standard cell, and guarantees a significantly higher efficiency in formation of reactive metabolites. The cell can be used in the ROXY EC system for off-line collection of metabolites. Flow rate and working potential can be optimized using the Dialogue software. The µ-PrepCell has a diamond film electrode which facilitates high working potentials up to 4.9 Volt for even the most demanding reaction conditions. The HyREF reference electrode is maintenance free and the stainless steel spacer is convenient to handle. In other words, the µ-PrepCell is designed for performance and offers a quick and clean alternative for in vitro methods.

µ-PrepCell and electrodes

u-PrepCell