Polyphenols in food

polyphenols

ALEXYS Polyphenols Analyzer

 

 

Flavonoids and flavanoids are naturally occurring antioxidant compounds belonging to the phytopolyphenols. Resveratrol, quercetin, and other polyphenolic flavonoids have been reported to be cancer preventive agents and are thought to have therapeutic importance in cardiovascular disease. These polyphenolic substances are found in wine, green tea, chocolate, cocoa and several other food products. The chromatogram is an example of 10X diluted Santa Digna Cabernet Sauvignon 2003, Chile. Peaks are: 1= Myricetin, 2= trans-Resveratrol, 3= Quercetin, 4= cis-Resveratrol, 5= Keampferol, EC= Epicatechin, EGC=epigallocatechin.

The ALEXYS Polyphenols Analyzer is an integrated system solution for the analysis of resveratrol, epicatechin, quercetin and other polyphenols in wine and food products. The system uses a gradient LC system to optimise separation.


Application Notes

Posters

System configuration

For the analysis of polyphenols in wine and food products a flowcell with glassy carbon electrode and HyREF was used. The HyREF is completely maintenance free, and compatible with high modifier percentages. The mobile phase contains up to 25% acetonitrile, therefore a 2mm column has been used at a flow rate of 200 uL/min which results in less chemical waste. The ALEXYS system comprises the following parts:

  • AS 110 autosampler, cool
  • LC 110S HPLC pump (2)
  • OR 110 organiser rack
  • Clarity data acquisition software
  • DECADE Elite electrochemical detector
  • Flow cell with GC WE, HyREF ALEXYS Analyzers

For further details see our product catalogue.

 

Part no Description
180.0094C ALEXYS Polyphenols Analyzer