Analysis of FDG

ALEXYS Analyzer for FDG




[18F]FDG is a radioactive glucose molecule used for PET imaging. It produces images that show the metabolic activity of tissues. In FDG-PET scanning, the high consumption of the sugar by tumor cells, as compared to the lower consumption by normal surrounding tissues, identifies these cells as cancer cells.

The ALEXYS analyzer for FDG provides a reliable solution for the quality control of FDG as required by the US and European Pharmacopeia (USP and EP). The DECADE Elite electrochemical detector in combination with a CarboPac column allows fast analysis of 8 minutes. The EP and USP methods are both evaluated and in compliance.

The EP and USP methods are based on isocratic separation using an anion exchange column and alkaline mobile phase (pH 13) followed by pulsed amperometric detection on a gold (Au) working electrode. An optimized pulse configuration is applied that results in less wear of the working electrode (less maintenance, longer lifetime of gold electrode). For the method evaluation an ALEXYS analyzer with an AS110 autosampler was used. In the case the number of samples per day is small, the ALEXYS Analyzer can be configured with a manual injection valve instead.

Application Notes

System configuration

For the analysis of FDG we selected the Flexcell electrochemical cell. This cell has an exchangeable working electrode which is particularly useful in pulsed electrochemical detection. The electrode material tends to vanish over the years, in the process. Exchanging an electrode disc only takes a few minutes. The HyREF is maintenance free even at pH 13! The ALEXYS Analyzer (180.0053E) comprises the following parts:

  • Injection valve (AS 110 autosampler is optional)
  • LC 110S HPLC pump
  • OR 110 organiser
  • Clarity data acquisition software
  • DECADE Elite electrochemical detector
  • Flexcell with Au WE, HyREF
  • HPLC column with pre-column
  • ALEXYS LC connection kit 
  • Polyethylene bottle kit



Part no Description
180.0053E ALEXYS Analyzer for FDG
191.0036L AS 110 autosampler standard 6p, LAN
250.1078 CarboPac PA20 analytical column, 150 x 3.0 mm ID
250.1079 CarboPac PA20 guard column, 50 x 3.0 mm ID